Ca2+ dynamics in the pancreatic islet
Functional networks in pancreatic islets
Simulated 3D islet of beta cells
Computational modeling of the islet function
Cell electrophysiology or the coupled β-cell model we use was adapted from the published Cha–Noma single-cell model where the change of the membrane potential with time for each β-cell is related to the sum of individual ion currents through that cell's membrane. This model modified with addition of the coupling current between the cells to form the coupled islet allows to predict Ca2+ and insulin secretion dynamics of the β-cell islet in-silico. We use the model to test how removal of specific cell subpopulations effects the function of the rest of the sells, to predict functional changes under diabetic conditions (reduced cell-cell coupling) and more.
Zebrafish image obtained with confocal microscopy
Paracrine and neural inputs in islets of Langerhans
We study to which extent paracrine (alpha-beta and delta-beta cell communication) forms the beta cell network. We are using pancreatic slices from human donors and mice in healthy and diabetic conditions.
Role of innervation in the formation of networks is studied in vivo in zebrafish models.